The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio).
Cryobiology
; 54(1): 121-4, 2007 Feb.
Article
en En
| MEDLINE
| ID: mdl-17217943
ABSTRACT
To identify a stage feasible for the cryopreservation of zebrafish oocytes, we investigated the permeability to water and cryoprotectants of immature (stage III) and mature (stage V) oocytes. The permeability to water (microm/min/atm) of immature oocytes at 25 degrees C (0.37) was significantly higher than that of mature oocytes (0.10). The permeability (x10(-3)cm/min) of immature oocytes to ethylene glycol, propylene glycol, and Me(2)SO (1.49-3.03) at 25 degrees C was substantially higher than that of mature oocytes approximately 0. The permeability of immature oocytes to glycerol was also high (1.75), although the permeability could not be measured in mature oocytes. Immature oocytes would be more suitable than mature oocytes for conservation of the zebrafish.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Pez Cebra
/
Agua
/
Criopreservación
/
Permeabilidad de la Membrana Celular
/
Crioprotectores
Límite:
Animals
Idioma:
En
Revista:
Cryobiology
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón