Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences.
Acta Trop
; 101(2): 91-4, 2007 Feb.
Article
en En
| MEDLINE
| ID: mdl-17276379
ABSTRACT
In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS1 differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS1 sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Clonorchis sinensis
/
Análisis de Secuencia de ADN
/
Genes de ARNr
/
ADN Espaciador Ribosómico
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Acta Trop
Año:
2007
Tipo del documento:
Article
País de afiliación:
China