[Production and identification of chicken egg yolk antibodies against soluble egg antigen of Schistosoma japonicum].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 25(3): 193-7, 2007 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-18038775
ABSTRACT
OBJECTIVE:
To produce and purify egg yolk immunoglobulin against soluble egg antigen (SEA) of Schistosoma japonicum, and evaluate its specificity and sensitivity.METHODS:
25-week old hen was intravenously and subcutaneously immunized with SEA of Schistosoma japonicum for 4 times. Each hen was first immunized with 60 microg SEA and subsequent injections were performed at 10-day intervals with 30 microg SEA. IgY was extracted from eggs of hen 35 d after the first inoculation by WD (water-dilution) method, eggs from non-immunized hen were used as negative control. The protein concentration of IgY was measured by BCA method, and IgY was analyzed by SDS-PAGE and Western blotting. SEA-based ELISA was used to evaluate the specificity and sensitivity of the IgY.RESULTS:
61 mg IgY was extracted from one egg. The results of SDS-PAGE and Western blotting demonstrated that the IgY contained one major protein band with molecular weight of 130,000 and could be recognized by SEA. Specific IgY could be immediately detected by SDS-PAGE and ELISA in the eggs laid by the hens from 10 days after the first immunization. On day 31 after the primary immunization, the antibody titer reached 11 600. 2.4 ng/ml SEA was detected by IgY based-sandwich ELISA, which indicated a high sensitivity of the purified IgY.CONCLUSION:
Anti-SEA IgY with high specificity and sensitivity has been obtained and purified.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Schistosoma japonicum
/
Inmunoglobulinas
/
Yema de Huevo
/
Antígenos Helmínticos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China