[Effects of endothelial lipase on mRNA expression of adhesion molecule of human umbilical vascular endothelial cells].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
; 19(12): 705-7, 2007 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-18093422
ABSTRACT
OBJECTIVE:
To explore the relationship between human umbilical vascular endothelial cells (HUVECs) and endothelial lipase (EL), and the effect of EL on expression of endothelial cell adhesion molecule (ICAM).METHODS:
HUVECs was treated with tumor necrosis factor-alpha(TNF-alpha) 10 microg/L and the mRNA of adhesion molecules [intercellular adhesion molecule-1 (ICAM-1), vascular cellular adhesion molecule-1 (VCAM-1) and E-selectin] were detected by reverse transcription-polymerase chain reaction (RT-PCR). Then the effect of 50 microg/L anti-endothelial lipase (anti-EL) antibody on the influence of TNF-alpha on these adhesion molecules was observed.RESULTS:
After being treated with TNF-alpha, the mRNA of adhesion molecules expressed by HUVECs were significant up-regulated, there was significant difference compared with control group (all P<0.01). These effects of TNF-alpha were significantly abolished by 50 microg/L anti-EL antibody (P<0.05 or P<0.01).CONCLUSION:
EL can affect the expression of adhesion molecules on endothelial cell adhesion molecule. This effect of EL may play a role in the pathophysiologic process in the pathogenesis progress of atherosclerosis.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Molécula 1 de Adhesión Intercelular
/
Molécula 1 de Adhesión Celular Vascular
/
Selectina E
/
Células Endoteliales
/
Lipasa
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
Asunto de la revista:
MEDICINA DE EMERGENCIA
/
TERAPIA INTENSIVA
Año:
2007
Tipo del documento:
Article
País de afiliación:
China