Characterization of an equine macrophage cell line: application to studies of EIAV infection.
Vet Microbiol
; 136(1-2): 8-19, 2009 Apr 14.
Article
en En
| MEDLINE
| ID: mdl-19038510
ABSTRACT
EIAV is a monocyte/macrophage tropic virus. To date, even though EIAV has been under investigation for numerous years, very few details have been elucidated about EIAV/macrophage interactions. This is largely due to the absence of an equine macrophage cell line that would support viral replication. Herein we describe the spontaneous immortalization and generation of a clonal equine macrophage-like (EML) cell line with the functional and immunophenotype characteristics of differentiated equine monocyte derived macrophage(s) (eMDM(s)). These cells possess strong non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles, and produce nitrites in response to LPS. The EML-3C cell line expresses the EIAV receptor for cellular entry (ELR1) and supports replication of the virulent EIAV(PV) biological clone. Thus, EML-3C cells provide a useful cell line possessing equine macrophage related properties for the growth and study of EIAV infection as well as of other equine macrophage tropic viruses.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Anemia Infecciosa Equina
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Virus de la Anemia Infecciosa Equina
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Caballos
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Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Portugal