High-throughput sequence analysis of variants of human cytomegalovirus strains Towne and AD169.
J Gen Virol
; 90(Pt 10): 2375-2380, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19553388
ABSTRACT
The genomes of commonly used variants of human cytomegalovirus (HCMV) strains Towne and AD169 each contain a substantial mutation in which a region (U(L)/b') at the right end of the long unique region has been replaced by an inverted duplication of a region from the left end of the genome. Using high-throughput technology, we have sequenced HCMV strain Towne (ATCC VR-977) and confirmed the presence of two variants, one exhibiting the replacement in U(L)/b' and the other intact in this region. Both variants are mutated in genes RL13, UL1, UL40, UL130, US1 and US9. We have also sequenced a novel AD169 variant (varUC) that is intact in U(L)/b' except for a small deletion that affects genes UL144, UL142, UL141 and UL140. Like other AD169 variants, varUC is mutated in genes RL5A, RL13, UL36 and UL131A. A subpopulation of varUC contains an additional deletion affecting genes IRS1, US1 and US2.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Variación Genética
/
Citomegalovirus
Límite:
Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Reino Unido