Circadian rhythm of trehalose in the face fly Musca autumnalis de Geer.
Chronobiol Int
; 7(5-6): 413-8, 1990.
Article
en En
| MEDLINE
| ID: mdl-2097074
ABSTRACT
Trehalose levels were determined over two 24 hr spans in groups of face fly adults 3-4 days after emergence from the puparium. Face fly pupae were placed in rearing chambers at 27 degrees C in a staggered light-dark regimen, LD 168, so that at a given clock hour, samples could be obtained at several different hours after lights on (HALO). Trehalose was determined in hemolymph collected from a puncture in the intersegmental membrane of the abdomen. Treated hemolymph samples were passed through a Bio-Rad Amino 5-S disaccharide column and a Waters 410 refractive index detector was used to differentiate among sugars. The circadian acrophase derived by cosinor analysis in hemolymph trehalose (when the values were 25.49 and 26.86 micrograms/microliters on the first and second days respectively) occurred at -226 degrees (ca 15 HALO) and the bathyphase at 24 HALO. The mesor = 11.82 micrograms/microliters trehalose, the amplitude = 8.57 micrograms/microliters trehalose and the P-value for presence of a rhythm was 0.003. Based on these data, differences between control and test flies in a bioassay of hypertrehalosemic activity would be most easily observed at 0-8 HALO, while exogenous hypotrehalosemic activity would be best assayed at 12-20 HALO.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Trehalosa
/
Muscidae
/
Ritmo Circadiano
Límite:
Animals
Idioma:
En
Revista:
Chronobiol Int
Asunto de la revista:
FISIOLOGIA
Año:
1990
Tipo del documento:
Article