Notoginsenoside ST-4 inhibits virus penetration of herpes simplex virus in vitro.
J Asian Nat Prod Res
; 13(6): 498-504, 2011 Jun.
Article
en En
| MEDLINE
| ID: mdl-21623512
ABSTRACT
Further study on steam-treated notoginseng, the roots of Panax notoginseng (Burk.) F.H. Chen (Araliaceae), which is a famous traditional Chinese medicine that is used both in raw and treated forms for a long time, led to the isolation of a new dammarane-type saponin, namely notoginsenoside ST-4. Its structure was elucidated to be 3ß,12ß,20(S)-tri-hydroxydammar-24-ene-3-O-ß-d-xylopyranosyl-(1 â 2)-ß-d-glucopyranosyl-(1 â 2)-ß-d-glu-copyranoside, based on the detailed analyses of the 1D and 2D NMR spectral data and acidic hydrolysis. Notoginsenoside ST-4 was investigated for its antiviral activity on herpes simplex type 1 (HSV-1) and type 2 (HSV-2) in vitro. The 50% effective concentration (EC(50)) values, determined by plaque reduction assay, were 16.47 ± 0.67 and 19.44 ± 1.16 µM for HSV-1 and HSV-2, respectively, whereas the 50% cytotoxic concentration (CC(50)) determined by the XTT test on Vero cells was 510.64 ± 4.56 µM. As analyzed by attachment assay and penetration assay based on plaque reduction assay, the antiviral activity of notoginsenoside ST-4 was principally due to the penetration inhibition effects, which was confirmed by fluorescence microscopy observation that notoginsenoside ST-4 blocked the penetration of virus. Therefore, notoginsenoside ST-4 might be a promising agent for herpes simplex virus infection.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Antivirales
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Saponinas
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Triterpenos
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Medicamentos Herbarios Chinos
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Simplexvirus
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Panax
Límite:
Animals
Idioma:
En
Revista:
J Asian Nat Prod Res
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
China