Structure-guided engineering of human thymidine kinase 2 as a positron emission tomography reporter gene for enhanced phosphorylation of non-natural thymidine analog reporter probe.

Campbell, Dean O; Yaghoubi, Shahriar S; Su, Ying; Lee, Jason T; Auerbach, Martin S; Herschman, Harvey; Satyamurthy, Nagichettiar; Czernin, Johannes; Lavie, Arnon; Radu, Caius G

Campbell, Dean O; Yaghoubi, Shahriar S; Su, Ying; Lee, Jason T; Auerbach, Martin S; Herschman, Harvey; Satyamurthy, Nagichettiar; Czernin, Johannes; Lavie, Arnon; Radu, Caius G.

Afiliación

Campbell DO; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095.
Yaghoubi SS; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095; CellSight Technologies, San Francisco, California 94107.
Su Y; Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, Illinois 60607.
Lee JT; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095.
Auerbach MS; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095.
Herschman H; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Department of Biological Chemistry, UCLA, Los Angeles, California 90095.
Satyamurthy N; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095.
Czernin J; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095.
Lavie A; Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, Chicago, Illinois 60607. Electronic address: Lavie@uic.edu.
Radu CG; Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, California, 90095; Ahmanson Translational Imaging Division, UCLA, Los Angeles, California, 90095. Electronic address: CRadu@mednet.ucla.edu.

J Biol Chem ; 287(1): 446-454, 2012 Jan 02.

Article en En | MEDLINE | ID: mdl-22074768

Asunto(s)

Genes Reporteros/genética; Tomografía de Emisión de Positrones/métodos; Ingeniería de Proteínas/métodos; Timidina Quinasa/química; Timidina Quinasa/genética; Timidina/análogos & derivados; Timidina/metabolismo; Adulto; Animales; Arabinofuranosil Uracilo/análogos & derivados; Arabinofuranosil Uracilo/química; Arabinofuranosil Uracilo/metabolismo; Arabinofuranosil Uracilo/farmacocinética; Femenino; Radioisótopos de Flúor; Guanina/análogos & derivados; Guanina/química; Guanina/metabolismo; Guanina/farmacocinética; Herpesvirus Humano 1/enzimología; Herpesvirus Humano 1/genética; Humanos; Masculino; Ratones; Persona de Mediana Edad; Modelos Moleculares; Fosforilación; Conformación Proteica; Timidina/farmacocinética; Timidina Quinasa/metabolismo

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Timidina / Timidina Quinasa / Ingeniería de Proteínas / Genes Reporteros / Tomografía de Emisión de Positrones Tipo de estudio: Prognostic_studies Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: J Biol Chem Año: 2012 Tipo del documento: Article

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