Library-free methylation sequencing with bisulfite padlock probes.
Nat Methods
; 9(3): 270-2, 2012 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-22306810
ABSTRACT
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sulfitos
/
ADN
/
Sondas de ADN
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos