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Pentamethylquercetin generates beneficial effects in monosodium glutamate-induced obese mice and C2C12 myotubes by activating AMP-activated protein kinase.
Shen, J Z; Ma, L N; Han, Y; Liu, J X; Yang, W Q; Chen, L; Liu, Y; Hu, Y; Jin, M W.
Afiliación
  • Shen JZ; Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei 430030, China.
Diabetologia ; 55(6): 1836-46, 2012 Jun.
Article en En | MEDLINE | ID: mdl-22415589
ABSTRACT
AIMS/

HYPOTHESIS:

Pentamethylquercetin (PMQ) has recently been shown to have glucose-lowering properties. Here, we aimed to characterise the effectiveness and underlying mechanisms of PMQ for ameliorating metabolic disorders in vivo and vitro.

METHODS:

We generated a mouse model of obesity by neonatal administration of monosodium glutamate (MSG) and used it to assess the properties of PMQ as a treatment for metabolic disorders. We also investigated the possible underlying mechanisms of PMQ in the prevention of metabolic disorders.

RESULTS:

Compared with normal mice, MSG mice had metabolic disorders, including central obesity, hyperinsulinaemia, insulin resistance, hyperglycaemia, hyperlipidaemia, decreased phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), and downregulated levels of GLUT4 in gastrocnemius muscles. In MSG mice, PMQ treatment (5, 10, 20 mg/kg daily) reduced body weight gain, waist circumference, adipose tissue mass, serum glucose, triacylglycerol and total cholesterol, while improving insulin resistance, activating AMPK and increasing ACC phosphorylation and GLUT4 abundance. In C2C12 myotubes, PMQ (10 µmol/l) increased glucose consumption by ∼65%. PMQ treatment (1-10 µmol/l) also activated AMPK, increased ACC phosphorylation and GLUT4 abundance, and upregulated the expression of some key genes involved in fatty acid oxidation. CONCLUSIONS/

INTERPRETATION:

These findings suggest that PMQ can ameliorate metabolic disorders at least in part via stimulation of AMPK activity.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Quercetina / Glutamato de Sodio / Proteínas Quinasas Activadas por AMP / Obesidad Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Diabetologia Año: 2012 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Quercetina / Glutamato de Sodio / Proteínas Quinasas Activadas por AMP / Obesidad Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Diabetologia Año: 2012 Tipo del documento: Article País de afiliación: China