A forward genetic screen identifies eukaryotic translation initiation factor 3, subunit H (eIF3h), as an enhancer of variegation in the mouse.
G3 (Bethesda)
; 2(11): 1393-6, 2012 Nov.
Article
en En
| MEDLINE
| ID: mdl-23173090
We have used a forward genetic screen to identify genes required for transgene silencing in the mouse. Previously these genes were found using candidate-based sequencing, a slow and labor-intensive process. Recently, whole-exome deep sequencing has accelerated our ability to find the causative point mutations, resulting in the discovery of novel and sometimes unexpected genes. Here we report the identification of translation initiation factor 3, subunit H (eIF3h) in two modifier of murine metastable epialleles (Mommes) lines. Mice carrying mutations in this gene have not been reported previously, and a possible involvement of eIF3h in transcription or epigenetic regulation has not been considered.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Subunidades de Proteína
/
Factor 3 de Iniciación Eucariótica
/
Efectos de la Posición Cromosómica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
G3 (Bethesda)
Año:
2012
Tipo del documento:
Article
País de afiliación:
Australia