[Effects of laser-assisted cochleostomy on inner hair cell ribbon synapse in rats].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 48(3): 237-40, 2013 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-23755791
ABSTRACT
OBJECTIVE:
To investigate the effects on ribbon synapse of inner hair cells after superpulsed CO2 laser-assisted cochleostomy in SD rats.METHODS:
Eighteen SD rats were randomly divided into laser-assisted surgery groups (2 W group and 5 W group), sham-operated group and control group. Ten of those were performed a cochleostomy using superpulsed CO2 laser with a corresponding power. Auditory brainstem responses (ABR) were measured pre-and postoperatively. The ribbon synapses at apical and middle cochlear turns were observed under laser scanning confocal microscope and then were quantified with 3ds Max software.RESULTS:
The postoperative ABR thresholds of the 2 W and 5 W groups were larger than the preoperative case (t = -5.65, P < 0.01; t = -4.97, P < 0.01). The synapse number at the middle turn decreased significantly in 5 W group (F = 17.15, P < 0.01), while no significant changes were noted at the apical turn (P > 0.05). There was no statistical difference in 2 W group (P > 0.05).CONCLUSIONS:
The superpulsed CO2 laser-assisted cochleostomy with high-power is accompanied by a synaptic injury, while no obvious effects after the low-power laser surgery, which might be a safe strategy to preform cochleostomy.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sinapsis
/
Cóclea
/
Láseres de Gas
/
Células Ciliadas Auditivas Internas
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
Año:
2013
Tipo del documento:
Article
País de afiliación:
China