Effect of ultrasound irradiation on bacterial internalization and bacteria-mediated gene transfer to cancer cells.
Ultrason Sonochem
; 21(3): 1187-93, 2014 May.
Article
en En
| MEDLINE
| ID: mdl-24373691
ABSTRACT
The present study demonstrates that ultrasound irradiation can facilitate bacteria-mediated gene delivery (bactofection). Escherichia coli modified with avidin were employed as a vehicle for delivery of the green fluorescent protein (GFP) gene, a model heterologous gene, into the breast cancer cell line MCF-7. Avidin-mediated binding of E. coli to MCF-7 cells enhanced the internalization of E. coli by approximately 17%, irrespective of the use of ultrasound irradiation. Furthermore, the use of ultrasound irradiation increased the internalization by approximately 5%, irrespective of the presence of avidin on the E. coli cell surface. The percentages of GFP-expressing MCF-7 cells at 24h after bactofection were below 0.5% and 2% for the case with only avidin-modification of E. coli cell surface and only ultrasound irradiation, respectively. However, combining avidin modification with the ultrasound treatment increased this value to 8%. Thus, the use of avidin-modified bacteria in conjunction with ultrasound irradiation has potential as an effective strategy for tumor-targeted bactofection.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ultrasonido
/
Transfección
/
Escherichia coli
Límite:
Humans
Idioma:
En
Revista:
Ultrason Sonochem
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2014
Tipo del documento:
Article
País de afiliación:
Japón