B-RAF kinase drives developmental axon growth and promotes axon regeneration in the injured mature CNS.
J Exp Med
; 211(5): 801-14, 2014 May 05.
Article
en En
| MEDLINE
| ID: mdl-24733831
ABSTRACT
Activation of intrinsic growth programs that promote developmental axon growth may also facilitate axon regeneration in injured adult neurons. Here, we demonstrate that conditional activation of B-RAF kinase alone in mouse embryonic neurons is sufficient to drive the growth of long-range peripheral sensory axon projections in vivo in the absence of upstream neurotrophin signaling. We further show that activated B-RAF signaling enables robust regenerative growth of sensory axons into the spinal cord after a dorsal root crush as well as substantial axon regrowth in the crush-lesioned optic nerve. Finally, the combination of B-RAF gain-of-function and PTEN loss-of-function promotes optic nerve axon extension beyond what would be predicted for a simple additive effect. We conclude that cell-intrinsic RAF signaling is a crucial pathway promoting developmental and regenerative axon growth in the peripheral and central nervous systems.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Axones
/
Transducción de Señal
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Sistema Nervioso Central
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Proteínas Proto-Oncogénicas B-raf
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Regeneración Nerviosa
Límite:
Animals
Idioma:
En
Revista:
J Exp Med
Año:
2014
Tipo del documento:
Article