Biosorption and biodegradation of pyrene by Brevibacillus brevis and cellular responses to pyrene treatment.
Ecotoxicol Environ Saf
; 115: 166-73, 2015 May.
Article
en En
| MEDLINE
| ID: mdl-25700095
ABSTRACT
Biodegradation has been proposed as an effective approach to remove pyrene, however, the information regarding cellular responses to pyrene treatment is limited thus far. In this study, the biodegradation and biosorption of pyrene by Brevibacillus brevis, along with cellular responses caused by pollutant were investigated by means of flow cytometry assay and scanning electron microscopy. The experimental results showed that pyrene was initially adsorbed by B. brevis and subsequently transported and intracellularly degraded. During this process, pyrene removal was primarily dependent on biodegradation. Cell invagination and cell surface corrugation occurred due to pyrene exposure. Nevertheless, cell regrowth after 96h treatment was observed, and the proportion of necrotic cell was only 2.8% after pyrene exposure for 120h, confirming that B. brevis could utilize pyrene as a sole carbon source for growth. The removal and biodegradation amount of pyrene (1mg/L) at 168h were 0.75 and 0.69mg/L, respectively, and the biosorption amount by inactivated cells was 0.41mg/L at this time.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pirenos
/
Contaminantes Químicos del Agua
/
Brevibacillus
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Año:
2015
Tipo del documento:
Article
País de afiliación:
China