miR-181a/b control the assembly of visual circuitry by regulating retinal axon specification and growth.
Dev Neurobiol
; 75(11): 1252-67, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-25728313
ABSTRACT
Connectivity and function of neuronal circuitry require the correct specification and growth of axons and dendrites. Here, we identify the microRNAs miR-181a and miR-181b as key regulators of retinal axon specification and growth. Loss of miR-181a/b in medaka fish (Oryzias latipes) failed to consolidate amacrine cell processes into axons and delayed the growth of retinal ganglion cell (RGC) axons. These alterations were accompanied by defects in visual connectivity and function. We demonstrated that miR-181a/b exert these actions through negative modulation of MAPK/ERK signaling that in turn leads to RhoA reduction and proper neuritogenesis in both amacrine cells and RGCs via local cytoskeletal rearrangement. Our results identify a new pathway for axon specification and growth unraveling a crucial role of miR-181a/b in the proper establishment of visual system connectivity and function.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Ganglionares de la Retina
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Axones
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Vías Visuales
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Células Amacrinas
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MicroARNs
Límite:
Animals
Idioma:
En
Revista:
Dev Neurobiol
Asunto de la revista:
BIOLOGIA
/
NEUROLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Italia