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Three R2R3-MYB transcription factors regulate distinct floral pigmentation patterning in Phalaenopsis spp.
Hsu, Chia-Chi; Chen, You-Yi; Tsai, Wen-Chieh; Chen, Wen-Huei; Chen, Hong-Hwa.
Afiliación
  • Hsu CC; Department of Life Sciences (C.-C.H., Y.-Y.C., H.-H.C.),Institute of Tropical Plant Sciences (W.-C.T.), andOrchid Research and Development Center (W.-C.T., W.-H.C., H.-H.C.), National Cheng Kung University, Tainan 701, Taiwan.
  • Chen YY; Department of Life Sciences (C.-C.H., Y.-Y.C., H.-H.C.),Institute of Tropical Plant Sciences (W.-C.T.), andOrchid Research and Development Center (W.-C.T., W.-H.C., H.-H.C.), National Cheng Kung University, Tainan 701, Taiwan.
  • Tsai WC; Department of Life Sciences (C.-C.H., Y.-Y.C., H.-H.C.),Institute of Tropical Plant Sciences (W.-C.T.), andOrchid Research and Development Center (W.-C.T., W.-H.C., H.-H.C.), National Cheng Kung University, Tainan 701, Taiwan.
  • Chen WH; Department of Life Sciences (C.-C.H., Y.-Y.C., H.-H.C.),Institute of Tropical Plant Sciences (W.-C.T.), andOrchid Research and Development Center (W.-C.T., W.-H.C., H.-H.C.), National Cheng Kung University, Tainan 701, Taiwan.
  • Chen HH; Department of Life Sciences (C.-C.H., Y.-Y.C., H.-H.C.),Institute of Tropical Plant Sciences (W.-C.T.), andOrchid Research and Development Center (W.-C.T., W.-H.C., H.-H.C.), National Cheng Kung University, Tainan 701, Taiwan hhchen@mail.ncku.edu.tw.
Plant Physiol ; 168(1): 175-91, 2015 May.
Article en En | MEDLINE | ID: mdl-25739699
ABSTRACT
Orchidaceae are well known for their fascinating floral morphologic features, specialized pollination, and distinctive ecological strategies. With their long-lasting flowers of various colors and pigmentation patterning, Phalaenopsis spp. have become important ornamental plants worldwide. In this study, we identified three R2R3-MYB transcription factors PeMYB2, PeMYB11, and PeMYB12. Their expression profiles were concomitant with red color formation in Phalaenopsis spp. flowers. Transient assay of overexpression of three PeMYBs verified that PeMYB2 resulted in anthocyanin accumulation, and these PeMYBs could activate the expression of three downstream structural genes Phalaenopsis spp. Flavanone 3-hydroxylase5, Phalaenopsis spp. Dihydroflavonol 4-reductase1, and Phalaenopsis spp. Anthocyanidin synthase3. In addition, these three PeMYBs participated in the distinct pigmentation patterning in a single flower, which was revealed by virus-induced gene silencing. In the sepals/petals, silencing of PeMYB2, PeMYB11, and PeMYB12 resulted in the loss of the full-red pigmentation, red spots, and venation patterns, respectively. Moreover, different pigmentation patterning was regulated by PeMYBs in the sepals/petals and lip. PeMYB11 was responsive to the red spots in the callus of the lip, and PeMYB12 participated in the full pigmentation in the central lobe of the lip. The differential pigmentation patterning was validated by RNA in situ hybridization. Additional assessment was performed in six Phalaenopsis spp. cultivars with different color patterns. The combined expression of these three PeMYBs in different ratios leads to a wealth of complicated floral pigmentation patterning in Phalaenopsis spp.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Pigmentación / Tipificación del Cuerpo / Orchidaceae / Flores Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article País de afiliación: Taiwán
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Pigmentación / Tipificación del Cuerpo / Orchidaceae / Flores Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Año: 2015 Tipo del documento: Article País de afiliación: Taiwán