Chemotaxonomic Studies of Nine Gentianaceae Species from Western China Based on Liquid Chromatography Tandem Mass Spectrometry and Fourier Transform Infrared Spectroscopy.
Phytochem Anal
; 27(3-4): 158-67, 2016 May.
Article
en En
| MEDLINE
| ID: mdl-26919544
ABSTRACT
INTRODUCTION:
Gentianaceae species which widely occur all over the world are used as folk medicine and raw food material with bitter properties. Although comparative analysis on metabolites in several Gentianaceae species has been reported, metabolic similarities used for chemotaxonomic studies are not yet clear.OBJECTIVE:
To systematically characterise the variations of holistic metabolome and characteristic metabolites (iridoid glycosides and phenols) in nine Gentianaceae species from western China.METHODOLOGY:
Fourier transform infrared (FT-IR) spectroscopy was applied to determine the variations of holistic metabolome. A targeted metabolic profiling using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was established for determination of seven characteristic metabolites and identification of their derivatives. Both FT-IR and LC-MS/MS data were subjected to chemometrics analysis for exploring variations in iridoid glycosides and phenols within these species.RESULTS:
Holistic metabolome in genera Gentiana and Swertia was largely different. Diversity of the biosynthetic pathway of iridoid glycosides was also observed in these species. Principal component analysis (PCA) showed a clear separation according to infrageneric classifications of genus Gentiana. Some secondary metabolites, such as mangiferin, rhodenthoside A-C, isoorientin, isovitexin, amarogentin, and swertianolin would serve as potential chemotaxonomic markers to differentiate Gentianaceae species. Furthermore, the accumulation of the six major metabolites seems to depend on geographical regions in Sect. Monopodiae and Sect. Cruciata.CONCLUSIONS:
The combination of LC-MS/MS and FT-IR would provide some potential evidence on chemotaxonomic studies of Gentianaceae. Copyright © 2016 John Wiley & Sons, Ltd.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fenoles
/
Extractos Vegetales
/
Espectroscopía Infrarroja por Transformada de Fourier
/
Gentianaceae
/
Espectrometría de Masas en Tándem
/
Metaboloma
/
Glicósidos Iridoides
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Phytochem Anal
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China