Functional Analysis of a Type-I Ribosome Inactivating Protein Balsamin from Momordica balsamina with Anti-Microbial and DNase Activity.
Plant Foods Hum Nutr
; 71(3): 265-71, 2016 Sep.
Article
en En
| MEDLINE
| ID: mdl-27319013
ABSTRACT
Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Momordica
/
Desoxirribonucleasas
/
Proteínas Inactivadoras de Ribosomas
/
Antibacterianos
Idioma:
En
Revista:
Plant Foods Hum Nutr
Asunto de la revista:
BOTANICA
/
CIENCIAS DA NUTRICAO
Año:
2016
Tipo del documento:
Article
País de afiliación:
Australia