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Transplantation of Airway Epithelial Stem/Progenitor Cells: A Future for Cell-Based Therapy.
Ghosh, Moumita; Ahmad, Shama; White, Carl W; Reynolds, Susan D.
Afiliación
  • Ghosh M; 1 Department of Pediatrics, National Jewish Health, Denver, Colorado.
  • Ahmad S; 2 Department of Anaesthesiology and Perioperative Medicine, University of Alabama, Birmingham, Alabama.
  • White CW; 3 Department of Pediatric Pulmonology, University of Colorado, Aurora, Colorado; and.
  • Reynolds SD; 4 Center for Perinatal Research, Nationwide Children's Hospital, Columbus, Ohio.
Am J Respir Cell Mol Biol ; 56(1): 1-10, 2017 01.
Article en En | MEDLINE | ID: mdl-27632244
Cell therapy has the potential to cure disease through replacement of malfunctioning cells. Although the tissue stem cell (TSC) is thought to be the optimal therapeutic cell, transplantation of TSC/progenitor cell mixtures has saved lives. We previously purified the mouse tracheobronchial epithelial TSCs and reported that in vitro amplification generated numerous TSCs. However, these cultures also contained TSC-derived progenitor cells and TSC repurification by flow cytometry compromised TSC self-renewal. These limitations prompted us to determine if a TSC/progenitor cell mixture would repopulate the injured airway epithelium. We developed a cell transplantation protocol and demonstrate that transplanted mouse and human tracheobronchial epithelial TSC/progenitor cell mixtures are 20-25% of airway epithelial cells, actively contribute to epithelial repair, and persist for at least 43 days. At 2 weeks after transplantation, TSCs/progenitor cells differentiated into the three major epithelial cell types: basal, secretory, and ciliated. We conclude that cell therapy that uses adult tracheobronchial TSCs/progenitor cells is an effective therapeutic option.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Madre / Trasplante de Células Madre / Células Epiteliales / Pulmón Tipo de estudio: Guideline Límite: Animals / Female / Humans Idioma: En Revista: Am J Respir Cell Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Madre / Trasplante de Células Madre / Células Epiteliales / Pulmón Tipo de estudio: Guideline Límite: Animals / Female / Humans Idioma: En Revista: Am J Respir Cell Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article