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Use of whole-genome sequencing data to analyze 23S rRNA-mediated azithromycin resistance.
Johnson, Steven R; Grad, Yonatan; Abrams, A Jeanine; Pettus, Kevin; Trees, David L.
Afiliación
  • Johnson SR; Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), US Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA. Electronic address: sbj1@cdc.gov.
  • Grad Y; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA; Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Abrams AJ; Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), US Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.
  • Pettus K; Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), US Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.
  • Trees DL; Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention (NCHHSTP), US Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.
Int J Antimicrob Agents ; 49(2): 252-254, 2017 Feb.
Article en En | MEDLINE | ID: mdl-28038960
ABSTRACT
The whole-genome sequences of 24 isolates of Neisseria gonorrhoeae with elevated minimum inhibitory concentrations (MICs) to azithromycin (≥2.0 µg/mL) were analyzed against a modified sequence derived from the whole-genome sequence of N. gonorrhoeae FA1090 to determine, by signal ratio, the number of mutant copies of the 23S rRNA gene and the copy number effect on 50S ribosome-mediated azithromycin resistance. Isolates that were predicted to contain four mutated copies were accurately identified compared with the results of direct sequencing. Fewer than four mutated copies gave less accurate results but were consistent with elevated MICs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN Bacteriano / ADN Ribosómico / ARN Ribosómico 23S / Análisis de Secuencia de ADN / Azitromicina / Antibacterianos / Neisseria gonorrhoeae Idioma: En Revista: Int J Antimicrob Agents Año: 2017 Tipo del documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: ADN Bacteriano / ADN Ribosómico / ARN Ribosómico 23S / Análisis de Secuencia de ADN / Azitromicina / Antibacterianos / Neisseria gonorrhoeae Idioma: En Revista: Int J Antimicrob Agents Año: 2017 Tipo del documento: Article