Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation.
Hereditas
; 154: 1, 2017.
Article
en En
| MEDLINE
| ID: mdl-28096780
ABSTRACT
BACKGROUND:
Tomatoes provide a significant dietary source of the carotenoids, lycopene and ß-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties.METHODS:
We crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel 'orange-brown' fruit color was identified.RESULTS:
We confirm two SNPs, one in CYC-B and another in SGR gene sequence, associated with segregation of 'orange-brown' fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high ß-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Clorofila
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Solanum lycopersicum
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Beta Caroteno
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Barajamiento de ADN
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Frutas
Idioma:
En
Revista:
Hereditas
Año:
2017
Tipo del documento:
Article