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Metabolic Profiling and Identification of Shikonins in Root Periderm of Two Invasive Echium spp. Weeds in Australia.
Skoneczny, Dominik; Weston, Paul A; Zhu, Xiaocheng; Gurr, Geoff M; Callaway, Ragan M; Barrow, Russel A; Weston, Leslie A.
Afiliación
  • Skoneczny D; Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. dskoneczny@csu.edu.au.
  • Weston PA; Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. pweston@csu.edu.au.
  • Zhu X; Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. xzhu@csu.edu.au.
  • Gurr GM; Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. ggurr@csu.edu.au.
  • Callaway RM; Institute of Applied Ecology, Fujian Agriculture & Forestry University, Fuzhou 350002, China. ggurr@csu.edu.au.
  • Barrow RA; Division of Biological Science, University of Montana, 32 Campus Dr, Missoula, MT 59812, USA. ray.callaway@mso.umt.edu.
  • Weston LA; Research School of Chemistry, Australian National University, Acton, ACT 2601, Australia. rab@anu.edu.au.
Molecules ; 22(2)2017 Feb 21.
Article en En | MEDLINE | ID: mdl-28230806
Metabolic profiling can be successfully implemented to analyse a living system's response to environmental conditions by providing critical information on an organism's physiological state at a particular point in time and allowing for both quantitative and qualitative assessment of a specific subset(s) of key metabolites. Shikonins are highly reactive chemicals that affect various cell signalling pathways and possess antifungal, antibacterial and allelopathic activity. Based on previous bioassay results, bioactive shikonins, are likely to play important roles in the regulation of rhizosphere interactions with neighbouring plants, microbes and herbivores. An effective platform allowing for rapid identification and accurate profiling of numerous structurally similar, difficult-to-separate bioactive isohexenylnaphthazarins (shikonins) was developed using UHPLC Q-TOF MS. Root periderm tissues of the invasive Australian weeds Echium plantagineum and its congener E. vulgare were extracted overnight in ethanol for shikonin profiling. Shikonin production was evaluated at seedling, rosette and flowering stages. Five populations of each species were compared for qualitative and quantitative differences in shikonin formation. Each species showed little populational variation in qualitative shikonin production; however, shikonin was considerably low in one population of E. plantagineum from Western New South Wales. Seedlings of all populations produced the bioactive metabolite acetylshikonin and production was upregulated over time. Mature plants of both species produced significantly higher total levels of shikonins and isovalerylshikonin > dimethylacrylshikonin > shikonin > acetylshikonin in mature E. plantagineum. Although qualitative metabolic profiles in both Echium spp. were nearly identical, shikonin abundance in mature plant periderm was approximately 2.5 times higher in perennial E. vulgare extracts in comparison to those of the annual E. plantagineum. These findings contribute to our understanding of the biosynthesis of shikonins in roots of two related invasive plants and their expression in relation to plant phenological stage.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Naftoquinonas / Raíces de Plantas / Echium / Metaboloma / Metabolómica / Malezas Tipo de estudio: Diagnostic_studies / Prognostic_studies / Qualitative_research País/Región como asunto: Oceania Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Naftoquinonas / Raíces de Plantas / Echium / Metaboloma / Metabolómica / Malezas Tipo de estudio: Diagnostic_studies / Prognostic_studies / Qualitative_research País/Región como asunto: Oceania Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Australia