Molecular characterisation, genetic variability and detection of a functional polymorphism influencing the promoter activity of OXT gene in goat and sheep.
J Dairy Res
; 84(2): 165-169, 2017 May.
Article
en En
| MEDLINE
| ID: mdl-28290268
ABSTRACT
The purpose of the study described in this Research Communication was to report the full characterisation of the goat and sheep oxytocin-neurophysin I gene (OXT), their promoters and amino acid sequences. Using the genomic DNA as template, we sequenced and compared the whole OXT gene (3 exons), plus 958/960 nucleotides at the 5' flanking region and 478/477 nucleotides at the 3' flanking region, in 46 sheep and 24 goats belonging to different breeds/genetic types reared in Italy, Greece and Germany. The comparison of the obtained sequences showed a high degree of genetic variability at these loci. In particular, we focused on the SNP g.438T > C as possible example of trans-specific polymorphism. This SNP alters a putative binding site of the transcription factor Oct-1. The set-up of a luciferase assay confirmed that the C variant of this SNP negatively affects the promoter activity of the sheep OXT gene. The results of this study suggest that the SNP g.438T > C might be useful to promote association studies with traits/physiological processes controlled by this hormone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cabras
/
Neurofisinas
/
Oxitocina
/
Ovinos
/
Regiones Promotoras Genéticas
/
Polimorfismo de Nucleótido Simple
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Dairy Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Italia