Unencumbered Pol ß lyase activity in nucleosome core particles.
Nucleic Acids Res
; 45(15): 8901-8915, 2017 Sep 06.
Article
en En
| MEDLINE
| ID: mdl-28911106
ABSTRACT
Packaging of DNA into the nucleosome core particle (NCP) is considered to exert constraints to all DNA-templated processes, including base excision repair where Pol ß catalyzes two key enzymatic steps:
5'-dRP lyase gap trimming and template-directed DNA synthesis. Despite its biological significance, knowledge of Pol ß activities on NCPs is still limited. Here, we show that removal of the 5'-dRP block by Pol ß is unaffected by NCP constraints at all sites tested and is even enhanced near the DNA ends. In contrast, strong inhibition of DNA synthesis is observed. These results indicate 5'-dRP gap trimming proceeds unperturbed within the NCP; whereas, gap filling is strongly limited. In the absence of additional factors, base excision repair in NCPs will stall at the gap-filling step.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ribosamonofosfatos
/
ADN
/
Nucleosomas
/
ADN Polimerasa beta
/
Reparación del ADN
/
Replicación del ADN
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos