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Molecular cloning and analysis of the regulation of cys-14+, a structural gene of the sulfur regulatory circuit of Neurospora crassa.
Ketter, J S; Marzluf, G A.
Afiliación
  • Ketter JS; Department of Biochemistry, Ohio State University, Columbus 43210.
Mol Cell Biol ; 8(4): 1504-8, 1988 Apr.
Article en En | MEDLINE | ID: mdl-2898097
The cys-14+ gene encodes sulfate permease II, which is primarily expressed in mycelia. cys-14+ is one of a set of sulfur-related structural genes under the control of cys-3+ and scon+, the regulatory genes of the sulfur control circuit. We have cloned cys-14+ from a cosmid library of Neurospora crassa DNA. A restriction fragment length polymorphism analysis showed that this clone maps to the region of chromosome IV corresponding to the cys-14+ locus. Northern blot analyses were used to examine the regulated expression of the cys-14+ gene. In the wild type, a 3-kilobase cys-14+ transcript was highly expressed under sulfur-derepressing conditions but completely absent during sulfur repression. A cys-3 mutant, which cannot synthesize any of the sulfur-controlled enzymes, including sulfate permease II, did not possess any cys-14+ transcript under either condition. A cys-3 temperature-sensitive revertant completely lacked any cys-14+ mRNA at the conditional temperature but expressed the cys-14+ transcript upon derepression at the permissive temperature. Mutation of a second sulfur regulatory gene, scon(c), causes the expression of sulfur-related enzymes in a constitutive fashion; the scon(c) mutant showed a corresponding constitutive expression of cys-14+ mRNA, such that it was present even in cells subjected to sulfur repression conditions. These results show that the cys-14+ gene is regulated through the modulation of message content by the cys-3+ and scon(c) control genes in response to the sulfur levels of the cells.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas Fúngicas / Regulación de la Expresión Génica / Clonación Molecular / Proteínas de Transporte de Anión / Genes / Genes Fúngicos / Proteínas de la Membrana / Neurospora / Neurospora crassa Idioma: En Revista: Mol Cell Biol Año: 1988 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas Fúngicas / Regulación de la Expresión Génica / Clonación Molecular / Proteínas de Transporte de Anión / Genes / Genes Fúngicos / Proteínas de la Membrana / Neurospora / Neurospora crassa Idioma: En Revista: Mol Cell Biol Año: 1988 Tipo del documento: Article