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Temporal changes in peritoneal cell phenotype and neoelastic matrix induction with hyaluronan oligomers and TGF-ß1 after implantation of engineered conduits.
Shojaee, Mozhgan; Swaminathan, Ganesh; Bashur, Chris A; Ramamurthi, Anand.
Afiliación
  • Shojaee M; Department of Biomedical Engineering, Florida Institute of Technology, Melbourne, FL, USA.
  • Swaminathan G; Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA.
  • Bashur CA; Department of Biomedical Engineering, Florida Institute of Technology, Melbourne, FL, USA.
  • Ramamurthi A; Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA.
J Tissue Eng Regen Med ; 12(6): 1420-1431, 2018 06.
Article en En | MEDLINE | ID: mdl-29701914
ABSTRACT
The neoassembly and maturation of elastic matrix is an important challenge for engineering small-diameter grafts for patients with peripheral artery disease. We have previously shown that hyaluronan oligomers and transforming growth factor-ß (elastogenic factors or EFs) promote elastogenesis in smooth muscle cell (SMC) culture. However, their combined effects on macrophages and inflammatory cells in vivo are unknown. This information is needed to use the body (e.g., peritoneal cavity) as an "in vivo bioreactor" to recruit autologous cells to implanted EF-functionalized scaffolds. In this study, we determined if peritoneal fluid cells respond to EFs like smooth muscle cells and if these responses differ between cells sourced during different stages of inflammation triggered by scaffold implantation. Electrospun poly(ε-caprolactone)/collagen conduits were implanted in the peritoneal cavity prior to peritoneal fluid collection at 3-42 days postimplantation. Cells from the fluid were cultured in vitro with and without EFs to determine their response. Their phenotype/behaviour was assessed with a DNA assay, quantitative real-time PCR, and immunofluorescence. The EFs reduced peritoneal cell proliferation, maintained cell contractility, and unexpectedly did not exhibit proelastic effects, which we attributed to differences in cell density. We found the greatest elastin deposition in regions containing a high cell density. Further, we found that cells isolated from the peritoneal cavity at longer times after conduit implantation responded better to the EFs and exhibited more CD31 expression than cells at an earlier time point. Overall, this study provides information about the potential use of EFs in vivo and can guide the design of future tissue-engineered vascular grafts.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Peritoneo / Ingeniería de Tejidos / Elasticidad / Factor de Crecimiento Transformador beta1 / Andamios del Tejido / Ácido Hialurónico Límite: Animals Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Peritoneo / Ingeniería de Tejidos / Elasticidad / Factor de Crecimiento Transformador beta1 / Andamios del Tejido / Ácido Hialurónico Límite: Animals Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos