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Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts.
Engel, Martin; Balez, Rachelle; Muñoz, Sonia Sanz; Cabral-da-Silva, Mauricio Castro; Stevens, Claire Helen; Bax, Monique; Do-Ha, Dzung; Sidhu, Kuldip; Sachdev, Perminder; Ooi, Lezanne.
Afiliación
  • Engel M; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Balez R; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Muñoz SS; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Cabral-da-Silva MC; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Stevens CH; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Bax M; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Do-Ha D; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Sidhu K; Centre for Healthy Brain Ageing School of Medicine, University of New South Wales, High Street, Kensington,. NSW, 2052, Australia.
  • Sachdev P; Centre for Healthy Brain Ageing School of Medicine, University of New South Wales, High Street, Kensington,. NSW, 2052, Australia.
  • Ooi L; Illawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: lezanne@uow.edu.au.
Stem Cell Res ; 32: 135-138, 2018 10.
Article en En | MEDLINE | ID: mdl-30278375
ABSTRACT
Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage. Resource table.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Piel / Reprogramación Celular / Células Madre Pluripotentes Inducidas / Fibroblastos Límite: Female / Humans / Middle aged Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: Australia
Texto completo
Imprimir
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Piel / Reprogramación Celular / Células Madre Pluripotentes Inducidas / Fibroblastos Límite: Female / Humans / Middle aged Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: Australia