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Efficient scarless genome editing in human pluripotent stem cells.
Ikeda, Kazuya; Uchida, Nobuko; Nishimura, Toshinobu; White, Joseph; Martin, Renata M; Nakauchi, Hiromitsu; Sebastiano, Vittorio; Weinberg, Kenneth I; Porteus, Matthew H.
Afiliación
  • Ikeda K; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Uchida N; ReGen Med Division, BOCO Silicon Valley, Palo Alto, CA, USA.
  • Nishimura T; Department of Genetics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
  • White J; Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
  • Martin RM; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Nakauchi H; Department of Genetics, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
  • Sebastiano V; Department of Obstetrics and Gynecology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA, USA.
  • Weinberg KI; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Porteus MH; Department of Pediatrics, Stanford University, Stanford, CA, USA. mporteus@stanford.edu.
Nat Methods ; 15(12): 1045-1047, 2018 12.
Article en En | MEDLINE | ID: mdl-30504872
ABSTRACT
Scarless genome editing in human pluripotent stem cells (hPSCs) represents a goal for both precise research applications and clinical translation of hPSC-derived therapies. Here we established a versatile and efficient method that combines CRISPR-Cas9-mediated homologous recombination with positive-negative selection of edited clones to generate scarless genetic changes in hPSCs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Genoma Humano / Células Madre Pluripotentes / ARN Interferente Pequeño / Células Madre Embrionarias / Recombinación Homóloga / Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Genoma Humano / Células Madre Pluripotentes / ARN Interferente Pequeño / Células Madre Embrionarias / Recombinación Homóloga / Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos