Albumin combined with Mannitol impairs whole blood coagulation and platelet function in vitro.
J Neurosurg Sci
; 65(6): 634-641, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-31079437
ABSTRACT
BACKGROUND:
The indication of hydroxyethyl starch is currently under critical discussion and albumin 5% (ALB) has an increasing use in the operating theatre. Therefore, ALB is routinely used in neurosurgical procedures and often combined with mannitol 20% (MAN). Purpose of this in-vitro study was to determine the influence of the combination of MAN and ALB on blood coagulation and platelet function.METHODS:
Twenty-two healthy volunteers were included into this study and 21 analyzed. Blood was obtained and diluted into five groups 1) 7% dilution with MAN; 2) 10% dilution with ALB; 3) 17% dilution with isotonic balanced electrolyte solution; 4) 17% dilution with MAN+ALB; and 5) undiluted blood as control group (CON). Rotational thrombelastometry via ROTEM® (EXTEM™/FIBTEM™ Test; SABIC, Riyad, Saudi Arabia) and thrombocyte aggregometry via Multiplate® (Roche Diagnostics, Grenzach-Wyhlen, Germany) (ASPI, ADP and TRAP-test) were used to detect differences within the intervention groups and compared to the control group.RESULTS:
The maximum clot firmness in the FIBTEM™ Test (SABIC) decreased under the normal range with the combination of MAN+ALB 8 mm (5.5-11) compared to CON 15 mm (12.5-20), P<0.05. Platelet function (ADP test) showed significant decreases for ALB 51 AUC (40-84) and MAN+ALB 54 AUC (41-68) compared to CON 92 AUC (75-101), P<0.05. Except in clotting time all other EXTEM™ tests (SABIC) of MAN+ALB subgroup showed significant impairment on blood coagulation compared to the control group.CONCLUSIONS:
In this in-vitro study clinically relevant dilutions of MAN+ALB showed a significant inhibition of blood coagulation and platelet function. Further in-vivo studies are necessary to confirm these results.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tromboelastografía
/
Manitol
Límite:
Humans
Idioma:
En
Revista:
J Neurosurg Sci
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania