SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM.
Commun Biol
; 2: 218, 2019.
Article
en En
| MEDLINE
| ID: mdl-31240256
ABSTRACT
Selecting particles from digital micrographs is an essential step in single-particle electron cryomicroscopy (cryo-EM). As manual selection of complete datasets-typically comprising thousands of particles-is a tedious and time-consuming process, numerous automatic particle pickers have been developed. However, non-ideal datasets pose a challenge to particle picking. Here we present the particle picking software crYOLO which is based on the deep-learning object detection system You Only Look Once (YOLO). After training the network with 200-2500 particles per dataset it automatically recognizes particles with high recall and precision while reaching a speed of up to five micrographs per second. Further, we present a general crYOLO network able to pick from previously unseen datasets, allowing for completely automated on-the-fly cryo-EM data preprocessing during data acquisition. crYOLO is available as a standalone program under http//sphire.mpg.de/ and is distributed as part of the image processing workflow in SPHIRE.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Programas Informáticos
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Microscopía por Crioelectrón
Idioma:
En
Revista:
Commun Biol
Año:
2019
Tipo del documento:
Article
País de afiliación:
Alemania