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Pro-angiogenesis effect and transcriptome profile of Shuxinyin formula in zebrafish.
Zhou, Zhong-Yan; Xiao, Ying; Zhao, Wai-Rong; Zhang, Jing; Shi, Wen-Ting; Ma, Zi-Lin; Ye, Qing; Chen, Xin-Lin; Tang, Nuo; Tang, Jing-Yi.
Afiliación
  • Zhou ZY; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China; State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macao.
  • Xiao Y; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Zhao WR; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China; Cardiac Rehabilitation Center of Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Zhang J; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Shi WT; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Ma ZL; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China; Cardiac Rehabilitation Center of Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Ye Q; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Chen XL; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China.
  • Tang N; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China. Electronic address: tangnuo2002@163.com.
  • Tang JY; Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China; Cardiac Rehabilitation Center of Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China. Electronic address: dr_tang@163.com.
Phytomedicine ; 65: 153083, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31600690
ABSTRACT

BACKGROUND:

Angiogenesis plays a critical role in ischemia disease like coronary heart disease. Shunxinyin formula has been developed for treating coronary heart disease according to the principle of traditional Chinese medicine while its underlying mechanism is not fully elucidated.

PURPOSE:

Here, we hypothesize Shuxinyin formula could promote angiogenesis and microcirculation, and the underlying mechanism is also investigated.

METHODS:

We established the chemical profile of Shuxinyin (SXY) extract utilizing a UHPLC-Q/Exactive analysis system and evaluated its pro-angiogenesis effect in zebrafish model. The underlying mechanisms were investigated by combination of pharmacological experiments with transcriptome analysis in zebrafish. Zebrafish treated with VEGF was served as the positive control in present study.

RESULTS:

We found SXY significantly enhanced the sub-intestinal vessel plexus (SIVs) growth in zebrafish. Co-treatment and post-treatment SXY attenuated VEGF receptor tyrosine kinase inhibitor II (VRI)-induced deficiency of intersegmental vessels (ISVs) in a concentration dependent manner. Post-treatment VEGF, which is a well-known angiogenesis driver, also partially ameliorated VRI-induced ISVs deficiency. In addition, SXY inhibited the down-regulation of VEGF receptors, including kdr, flt1 and kdrl, induced by VRI in zebrafish. The pro-angiogenesis effect of SXY on VRI-induced ISVs deficiency was suppressed by PI3K and JNK inhibitors, and Akt inhibitor abolished the pro-angiogenesis effect of SXY. The transcriptome profile of SXY preventing from VRI-induced vascular growth deficiency revealed that the underlying mechanisms were also co-related to cell junction, apoptosis and autophagy.

CONCLUSION:

We could conclude that SXY presented pro-angiogenesis effect and the action mechanisms were involved in VEGF/PI3K/Akt/MAPK signaling pathways, cell junction, apoptosis and autophagy.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pez Cebra / Medicamentos Herbarios Chinos / Inductores de la Angiogénesis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Phytomedicine Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2019 Tipo del documento: Article País de afiliación: Macao

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pez Cebra / Medicamentos Herbarios Chinos / Inductores de la Angiogénesis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Phytomedicine Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2019 Tipo del documento: Article País de afiliación: Macao