Transcriptome analysis and safety profile of the early-phase clinical response to an adjuvanted grass allergoid immunotherapy.
World Allergy Organ J
; 12(11): 100087, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31768216
ABSTRACT
BACKGROUND:
Specific immunotherapy is the only type of disease-modifying treatment, which induces rapid desensitization and long-term sustained unresponsiveness in patients with seasonal allergic rhinoconjunctivitis. The safety and tolerability of a new cumulative dose regimen of 35600 SU Grass MATA MPL for subcutaneous immunotherapy were assessed in pre-seasonal, single-blind, placebo controlled Phase I clinical study. Underlying immunological mechanisms were explored using transcriptome analysis of peripheral blood mononuclear cells.METHODS:
Study subjects with a history of moderate to severe seasonal allergic rhinitis and/or conjunctivitis (SAR) due to grass (Pooideae) pollen exposure were randomized on a 11 ratio to receive either six 1.0 mL injections of cumulative dose regimen 35600 SU of Grass MATA MPL or placebo. The study consisted of three periods screening, randomization and treatment and End of Study period. Blood samples were taken for clinical safety laboratory assessments and for the assessment of gene expression analysis during screening visit and End of Study visit. The safety statistics was calculated using Fisher's exact test. Delta Delta Ct method analysis of RT2 Profiler PCR Array gene expression results was used to calculate changes in gene expression level. Genes with the absolute value of log2 fold change greater than ±1.1 and p-value less than 0.05 were identified as differentially expressed and underwent IPA data analysis.RESULTS:
The results of the study indicated that the higher cumulative dose regimen of the immunotherapy was well-tolerated. Changes in gene expression profile were associated with early immune responses implicating innate and adaptive immune mechanisms. Pathways and mechanistic network analysis via IPA mapped differentially expressed genes onto canonical pathways related to T cell differentiation, cytokine signalling and Th1/Th2 activation pathways. The transcriptome findings of the study could be further verified in large-scale field studies in order to explore their potential as predictive markers of successful immunotherapy.CONCLUSIONS:
The higher dose cumulative regime 35600 SU of Grass MATA MPL vaccine was well tolerated and safe. Molecular markers IL-27, IL-10, IL-4, TNF, IFNγ, TGFß and TLR4 were the main predicted molecular drivers of the observed gene expression changes following early stages of SIT with Grass MATA MPL immunotherapy.
ADRs, adverse drug reactions; AE, adverse events; AIT, allergen mmunotherapy; ARC, adverse reaction complex; Allergen immunotherapy; Allergoid; DC, dendritic cell; EAACI, European Academy of Allergy and Clinical Immunology; FEV, forced expiratory volume; FVC, forced vital capacity; Grass pollen; IPA, Ingenuity Pathway Analysis; MATA, modified allergen tyrosine adsorbate; MCT, microcrystalline tyrosine; MPL, monophosphoryl lipid A; SAEs, serious adverse events; SAR, seasonal allergic rhinoconjunctivitis; SD, standard deviation; SIT, specific immunotherapy; SU, standardized units; Safety; TEAEs, treatment-emergent adverse events; TLR, toll-like receptor; TSS, total symptom score; Transcriptome; URA, Upstream Regulator Analysis; mRNA, messenger ribosomal nucleic acid
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Tipo de estudio:
Clinical_trials
/
Prognostic_studies
Idioma:
En
Revista:
World Allergy Organ J
Año:
2019
Tipo del documento:
Article
País de afiliación:
Reino Unido