Examination of the human motor endplate after brachial plexus injury with two-photon microscopy.
Muscle Nerve
; 61(3): 390-395, 2020 03.
Article
en En
| MEDLINE
| ID: mdl-31820462
ABSTRACT
INTRODUCTION:
After traumatic nerve injury, neuromuscular junction remodeling plays a key role in determining functional outcomes. Immunohistochemical analyses of denervated muscle biopsies may provide valuable prognostic data regarding clinical outcomes to supplement electrodiagnostic studies.METHODS:
We performed biopsies on nonfunctioning deltoid muscles in two patients after gunshot wounds and visualized the neuromuscular junctions using two-photon microscopy with immunohistochemistry.RESULTS:
Although the nerves in both patients showed evidence of acute Wallerian degeneration, some of the motor endplates were intact but exhibited significantly decreased surface area and volume. Both patients exhibited substantial recovery of motor function over several weeks postinjury.DISCUSSION:
Two-photon microscopic assessment of neuromuscular junction integrity and motor endplate morphometry in muscle biopsies provided evidence of partial sparing of muscle innervation. This finding supported the clinical judgment that eventual recovery would occur. With further study, this technique may help to guide operative decisionmaking after traumatic nerve injuries.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neuropatías del Plexo Braquial
/
Placa Motora
Tipo de estudio:
Prognostic_studies
/
Qualitative_research
Límite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Muscle Nerve
Año:
2020
Tipo del documento:
Article