Prevention and Treatment of Pseudomonas Aeruginosa-Based Biofilm with Ethanol.
Isr Med Assoc J
; 22(5): 299-302, 2020 May.
Article
en En
| MEDLINE
| ID: mdl-32378822
ABSTRACT
BACKGROUND:
Although indwelling catheters are increasingly used in modern medicine, they can be a source of microbial contamination and hard-to-treat biofilms, which jeopardize patient lives. At times 70% ethanol is used as a catheter-lock solution due to its bactericidal properties. However, high concentrations of ethanol can result in adverse effects and in malfunction of the catheters.OBJECTIVES:
To determine whether low concentrations of ethanol can prevent and treat biofilms of Pseudomonas aeruginosa.METHODS:
Ethanol was tested at a concentration range of 0.625-80% against laboratory and clinical isolates of P. aeruginosa for various time periods (2-48 hours). The following parameters were evaluated following ethanol exposure prevention of biofilm formation, reduction of biofilm metabolic activity, and inhibition of biofilm regrowth.RESULTS:
Exposing P. aeruginosa to twofold ethanol gradients demonstrated a significant biofilm inhibition at concentrations as low as 2.5%. Treating pre-formed biofilms of P. aeruginosa with 20% ethanol for 4 hours caused a sharp decay in the metabolic activity of both the laboratory and clinical P. aeruginosa isolates. In addition, treating mature biofilms with 20% ethanol prevented the regrowth of bacteria encased within it.CONCLUSIONS:
Low ethanol concentrations (2.5%) can prevent in vitro biofilm formation of P. aeruginosa. Treatment of previously formed biofilms can be achieved using 20% ethanol, thereby keeping the catheters intact and avoiding complications that can result from high ethanol concentrations.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pseudomonas aeruginosa
/
Biopelículas
/
Etanol
/
Antiinfecciosos Locales
Límite:
Humans
Idioma:
En
Revista:
Isr Med Assoc J
Asunto de la revista:
MEDICINA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Israel