IgM and IgA in addition to IgG autoantibodies against FcÉRIα are frequent and associated with disease markers of chronic spontaneous urticaria.
Allergy
; 75(12): 3208-3215, 2020 12.
Article
en En
| MEDLINE
| ID: mdl-32446275
ABSTRACT
BACKGROUND:
IgG autoantibodies against the high-affinity IgE receptor, FcÉRIα, contribute the pathogenesis of autoimmune chronic spontaneous urticaria (CSU). However, it is not known whether such patients also exhibit IgM or IgA autoantibodies against FcÉRIα. To address this question we developed an ELISA to assess serum levels of IgG, IgM, and IgA autoantibodies against FcÉRIα and investigated whether their presence is linked to clinical features of CSU including the response to autologous serum skin testing (ASST).METHODS:
Serum samples of 35 CSU patients (25 ASST-positive) and 52 healthy control individuals were analyzed using a newly developed competitive ELISA for IgG, IgM, and IgA autoantibodies to FcÉRIα.RESULTS:
One in four CSU patients (8/35, 24%) had elevated serum levels of IgG-anti-FcÉRIα compared with (3/52, 6%) healthy controls. More than half of patients had IgM (21/35, 60%) and IgA (20/35, 57%) vs (3/52, 5%) each in healthy controls. Elevated IgM, but not IgG or IgA, autoantibodies were significantly more frequent in ASST-positive CSU patients (18/25, 72%) compared with ASSTnegative patients (3/10, 33%, P = .022). Also, elevated levels of IgM-anti-FcÉRIα, but not of IgG or IgA against FcÉRIα, were linked to low blood basophil (r = .414, P = .021) and eosinophil (r = .623, P < .001) counts.CONCLUSIONS:
Increased serum levels of IgM-anti-FcÉRIα are common in patients with CSU and linked to features of autoimmune CSU. The role and relevance of autoantibodies to FcÉRIα in CSU can and should be further characterized in future studies, and our novel assay can help with this.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Urticaria
/
Urticaria Crónica
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Allergy
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania