In Vitro Characterization of Sumoylation of HR Proteins.
Methods Mol Biol
; 2153: 483-502, 2021.
Article
en En
| MEDLINE
| ID: mdl-32840800
ABSTRACT
In vitro analysis of posttranslational modifications such as sumoylation provides a great tool to not only identify the target proteins but also to characterize the specific effects of this modification on the protein features and uncover possible regulatory mechanism. In this chapter, we will describe the purification of yeast SUMO machinery proteins and their use to identify SUMO modification of target proteins in vitro. Furthermore, we will show several examples characterizing the effect of sumoylation on the biochemical activities of various proteins involved in homologous recombination (HR) that helped to better understand the regulatory role of this modification.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina
/
Complejos de Ubiquitina-Proteína Ligasa
/
Escherichia coli
/
Recombinación Homóloga
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
República Checa