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Integrated Label-Free and 10-Plex DiLeu Isobaric Tag Quantitative Methods for Profiling Changes in the Mouse Hypothalamic Neuropeptidome and Proteome: Assessment of the Impact of the Gut Microbiome.
Liu, Rui; Wei, Pingli; Keller, Caitlin; Orefice, Nicola Salvatore; Shi, Yatao; Li, Zihui; Huang, Junfeng; Cui, Yusi; Frost, Dustin C; Han, Shuying; Cross, Tzu-Wen L; Rey, Federico E; Li, Lingjun.
Afiliación
  • Liu R; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Wei P; School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, P. R. China.
  • Keller C; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing 210023, P. R. China.
  • Orefice NS; Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, P. R. China.
  • Shi Y; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
  • Li Z; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
  • Huang J; Department of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Cui Y; Waisman Center, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Frost DC; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Han S; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
  • Cross TL; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Rey FE; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
  • Li L; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin 53705, United States.
Anal Chem ; 92(20): 14021-14030, 2020 10 20.
Article en En | MEDLINE | ID: mdl-32926775
Gut microbiota can regulate host physiological and pathological status through gut-brain communications or pathways. However, the impact of the gut microbiome on neuropeptides and proteins involved in regulating brain functions and behaviors is still not clearly understood. To address the problem, integrated label-free and 10-plex DiLeu isobaric tag-based quantitative methods were implemented to compare the profiling of neuropeptides and proteins in the hypothalamus of germ-free (GF)- vs conventionally raised (ConvR)-mice. A total of 2943 endogenous peptides from 63 neuropeptide precursors and 3971 proteins in the mouse hypothalamus were identified. Among these 368 significantly changed peptides (fold changes over 1.5 and a p-value of <0.05), 73.6% of the peptides showed higher levels in GF-mice than in ConvR-mice, and 26.4% of the peptides had higher levels in ConvR-mice than in GF-mice. These peptides were mainly from secretogranin-2, phosphatidylethanolamine-binding protein-1, ProSAAS, and proenkephalin-A. A quantitative proteomic analysis employing DiLeu isobaric tags revealed that 282 proteins were significantly up- or down-regulated (fold changes over 1.2 and a p-value of <0.05) among the 3277 quantified proteins. These neuropeptides and proteins were mainly involved in regulating behaviors, transmitter release, signaling pathways, and synapses. Interestingly, pathways including long-term potentiation, long-term depression, and circadian entrainment were involved. In the present study, a combined label-free and 10-plex DiLeu-based quantitative method enabled a comprehensive profiling of gut microbiome-induced dynamic changes of neuropeptides and proteins in the hypothalamus, suggesting that the gut microbiome might mediate a range of behavioral changes, brain development, and learning and memory through these neuropeptides and proteins.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neuropéptidos / Proteoma / Microbioma Gastrointestinal / Hipotálamo / Leucina Límite: Animals Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neuropéptidos / Proteoma / Microbioma Gastrointestinal / Hipotálamo / Leucina Límite: Animals Idioma: En Revista: Anal Chem Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos