Ultrasensitive Detection of the Hepatitis E Virus by Electrocatalytic Water Oxidation Using Pt-Co3O4 Hollow Cages.
ACS Appl Mater Interfaces
; 12(45): 50212-50221, 2020 Nov 11.
Article
en En
| MEDLINE
| ID: mdl-32967416
ABSTRACT
A sensitive virus detection method applicable for an early stage increases the probability of survival. Here, we develop a simple and rapid detection strategy for the detection of the hepatitis E virus (HEV) by an electrocatalytic water oxidation reaction (WOR) using a platinum (Pt)-incorporated cobalt (Co)-based zeolite imidazole framework (ZIF-67). The surface cavity of ZIF-67 enables the rich loading of Pt NPs, and subsequent calcination etches the cavity, promoting the electrocatalytic activity of Pt-Co3O4 HCs. The Pt-Co3O4 HCs show excellent behavior for the WOR due to the synergistic interaction of Pt and Co3O4, evaluated by voltammetry and chronoamperometry. The synthesized Pt-Co3O4 HCs are conjugated with anti-HEV antibody (Ab@Pt-Co3O4 HCs); the electrocatalytic activity of Ab@Pt-Co3O4 HCs is combined with that of antibody-conjugated magnetic nanoparticles (MNPs) for HEV detection by a magneto-and-nanocomposite sandwich immunoassay. The sensor is challenged to detect the HEV in spiked serum samples and HEV G7 genotypes collected from the cell culture supernatant, reaching a low limit of detection down to 61 RNA copies mL-1. This work establishes a free-indicator one-step approach with the controlled design of Pt-Co3O4 HCs, which presents an effective WOR technique for virus detection in a neutral pH solution, which can be extended to electrocatalytic studies in the future integrated biosensing systems.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Óxidos
/
Platino (Metal)
/
Agua
/
Técnicas Biosensibles
/
Virus de la Hepatitis E
/
Cobalto
/
Técnicas Electroquímicas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón