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Variation in tumor pH affects pH-triggered delivery of peptide-modified magnetic nanoparticles.
Pershina, Alexandra G; Brikunova, Olga Ya; Demin, Alexander M; Abakumov, Maxim A; Vaneev, Alexander N; Naumenko, Victor A; Erofeev, Alexander S; Gorelkin, Peter V; Nizamov, Timur R; Muslimov, Albert R; Timin, Alexander S; Malkeyeva, Dina; Kiseleva, Elena; Vtorushin, Sergey V; Larionova, Irina V; Gereng, Elena A; Minin, Artem S; Murzakaev, Aidar M; Krasnov, Victor P; Majouga, Alexander G; Ogorodova, Ludmila M.
Afiliación
  • Pershina AG; Siberian State Medical University, Tomsk, Russia; Research School of Chemical and Biomedical Engineering, National Research Tomsk Polytechnic University, Tomsk, Russia. Electronic address: allysyz@mail.ru.
  • Brikunova OY; Siberian State Medical University, Tomsk, Russia.
  • Demin AM; Postovsky Institute of Organic Synthesis UB RAS, Yekaterinburg, Russia.
  • Abakumov MA; National University of Science and Technology MISiS, Moscow, Russia.
  • Vaneev AN; National University of Science and Technology MISiS, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia.
  • Naumenko VA; National University of Science and Technology MISiS, Moscow, Russia; V. Serbsky National Medical Research Center for Psychiatry and Narcology, Moscow, Russia.
  • Erofeev AS; National University of Science and Technology MISiS, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia.
  • Gorelkin PV; National University of Science and Technology MISiS, Moscow, Russia; Medical Nanotechnology LLC, Moscow, Russia.
  • Nizamov TR; National University of Science and Technology MISiS, Moscow, Russia.
  • Muslimov AR; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia.
  • Timin AS; Research School of Chemical and Biomedical Engineering, National Research Tomsk Polytechnic University, Tomsk, Russia; Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia.
  • Malkeyeva D; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
  • Kiseleva E; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
  • Vtorushin SV; Siberian State Medical University, Tomsk, Russia; Cancer Research Institute, Tomsk National Research Medical Center RAS, Tomsk, Russia.
  • Larionova IV; Cancer Research Institute, Tomsk National Research Medical Center RAS, Tomsk, Russia; National Research Tomsk State University, Tomsk, Russia.
  • Gereng EA; Siberian State Medical University, Tomsk, Russia.
  • Minin AS; Mikheev Institute of Metal Physics UB RAS, Yekaterinburg, Russia.
  • Murzakaev AM; Institute of Electrophysics UB RAS, Yekaterinburg, Russia; Ural Federal University, Yekaterinburg, Russia.
  • Krasnov VP; Postovsky Institute of Organic Synthesis UB RAS, Yekaterinburg, Russia; Ural Federal University, Yekaterinburg, Russia.
  • Majouga AG; National University of Science and Technology MISiS, Moscow, Russia; Lomonosov Moscow State University, Moscow, Russia; Dmitry Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
  • Ogorodova LM; Siberian State Medical University, Tomsk, Russia.
Nanomedicine ; 32: 102317, 2021 02.
Article en En | MEDLINE | ID: mdl-33096245
Acidification of the extracellular matrix, an intrinsic characteristic of many solid tumors, is widely exploited for physiologically triggered delivery of contrast agents, drugs, and nanoparticles to tumor. However, pH of tumor microenvironment shows intra- and inter-tumor variation. Herein, we investigate the impact of this variation on pH-triggered delivery of magnetic nanoparticles (MNPs) modified with pH-(low)-insertion peptide (pHLIP). Fluorescent flow cytometry, laser confocal scanning microscopy and transmission electron microscopy data proved that pHLIP-conjugated MNPs interacted with 4T1 cells in two-dimensional culture and in spheroids more effectively at pH 6.4 than at pH 7.2, and entered the cell via clathrin-independent endocytosis. The accumulation efficiency of pHLIP-conjugated MNPs in 4T1 tumors after their intravenous injection, monitored in vivo by magnetic resonance imaging, showed variation. Analysis of the tumor pH profiles recorded with implementation of original nanoprobe pH sensor, revealed obvious correlation between pH measured in the tumor with the amount of accumulated MNPs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Nanopartículas de Magnetita / Microambiente Tumoral / Proteínas de la Membrana / Neoplasias Límite: Animals Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Nanopartículas de Magnetita / Microambiente Tumoral / Proteínas de la Membrana / Neoplasias Límite: Animals Idioma: En Revista: Nanomedicine Asunto de la revista: BIOTECNOLOGIA Año: 2021 Tipo del documento: Article