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Elongation factor eEF2 kinase and autophagy jointly promote survival of cancer cells.
Lenchine, Roman V; Rao, Sushma R; Wang, Xuemin; Fang, Danielle Meiwen; Proud, Christopher G.
Afiliación
  • Lenchine RV; Lifelong Health, South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Rao SR; The University of Adelaide, Adelaide, SA 5000, Australia.
  • Wang X; The University of Adelaide, Adelaide, SA 5000, Australia.
  • Fang DM; Proteomics, Metabolomics and MS-Imaging Facility, South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
  • Proud CG; Lifelong Health, South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.
Biochem J ; 478(8): 1547-1569, 2021 04 30.
Article en En | MEDLINE | ID: mdl-33779695
ABSTRACT
Cells within solid tumours can become deprived of nutrients; in order to survive, they need to invoke mechanisms to conserve these resources. Using cancer cells in culture in the absence of key nutrients, we have explored the roles of two potential survival mechanisms, autophagy and elongation factor 2 kinase (eEF2K), which, when activated, inhibits the resource-intensive elongation stage of protein synthesis. Both processes are regulated through the nutrient-sensitive AMP-activated protein kinase and mechanistic target of rapamycin complex 1 signalling pathways. We find that disabling both autophagy and eEF2K strongly compromises the survival of nutrient-deprived lung and breast cancer cells, whereas, for example, knocking out eEF2K alone has little effect. Contrary to some earlier reports, we find no evidence that eEF2K regulates autophagy. Unexpectedly, eEF2K does not facilitate survival of prostate cancer PC3 cells. Thus, eEF2K and autophagy enable survival of certain cell-types in a mutually complementary manner. To explore this further, we generated, by selection, cells which were able to survive nutrient starvation even when autophagy and eEF2K were disabled. Proteome profiling using mass spectrometry revealed that these 'resistant' cells showed lower levels of diverse proteins which are required for energy-consuming processes such as protein and fatty acid synthesis, although different clones of 'resistant cells' appear to adapt in dissimilar ways. Our data provide further information of the ways that human cells cope with nutrient limitation and to understanding of the utility of eEF2K as a potential target in oncology.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Regulación Neoplásica de la Expresión Génica / Ácido Pirúvico / Metabolismo Energético / Quinasa del Factor 2 de Elongación / Glucosa / Glutamina Límite: Humans Idioma: En Revista: Biochem J Año: 2021 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Autofagia / Regulación Neoplásica de la Expresión Génica / Ácido Pirúvico / Metabolismo Energético / Quinasa del Factor 2 de Elongación / Glucosa / Glutamina Límite: Humans Idioma: En Revista: Biochem J Año: 2021 Tipo del documento: Article País de afiliación: Australia