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Adaptation mechanism of aerobic denitrifier Enterobacter cloacae strain HNR to short-term ZnO nanoparticle stresses.
Ma, Teng-Fei; Chen, You-Peng; Yan, Peng; Fang, Fang; Shen, Yu; Mao, Zheng; Guo, Jin-Song; Zhao, Bin; Feng, Li.
Afiliación
  • Ma TF; National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, China; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China; Environmental Engineering
  • Chen YP; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China. Electronic address: ypchen@cqu.edu.cn.
  • Yan P; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
  • Fang F; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
  • Shen Y; National Research Base of Intelligent Manufacturing Service, Chongqing Technology and Business University, Chongqing, 400067, China; Chongqing South-to-Thais Environmental Protection Technology Research Institute Co., Ltd., Chongqing, 400069, China.
  • Mao Z; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
  • Guo JS; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
  • Zhao B; Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
  • Feng L; Environmental Engineering Technology Research Center, Chongqing Academy of Ecological and Environmental Sciences, Chongqing, 401147, China.
Environ Res ; 197: 111178, 2021 06.
Article en En | MEDLINE | ID: mdl-33865818
The adaptation mechanism of a wild type (WT) and resistant type (Re) strain of the aerobic denitrifier Enterobacter cloacae strain HNR to short-term ZnO nanoparticle (NP) stresses was investigated. The results showed that Re maintained higher nitrite reductase (NIR) and nitrate reductase (NR) activities and showed lower increment of reactive oxygen species (ROS) than WT, under ZnO NP stresses. The affinity constant (KA) of WT to Zn2+ was 5.06 times that of Re, indicating that Re was more repulsive to Zn2+ released by ZnO NPs. Transcriptomic analysis revealed that the up-regulation of the nitrogen metabolism of Re helped maintain NIR and NR activities, that the enhancement of purine metabolism lowered the intracellular ROS increment, and that the up-regulation of cationic antimicrobial peptide resistance contributed to the lower KA of Re to Zn2+. These findings provided new insights into the adaptation mechanism of aerobic denitrifying bacteria to ZnO NPs.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Óxido de Zinc / Nanopartículas Idioma: En Revista: Environ Res Año: 2021 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Óxido de Zinc / Nanopartículas Idioma: En Revista: Environ Res Año: 2021 Tipo del documento: Article