Fractalkine signaling regulates oligodendroglial cell genesis from SVZ precursor cells.
Stem Cell Reports
; 16(8): 1968-1984, 2021 08 10.
Article
en En
| MEDLINE
| ID: mdl-34270934
ABSTRACT
Neural and oligodendrocyte precursor cells (NPCs and OPCs) in the subventricular zone (SVZ) of the brain contribute to oligodendrogenesis throughout life, in part due to direct regulation by chemokines. The role of the chemokine fractalkine is well established in microglia; however, the effect of fractalkine on SVZ precursor cells is unknown. We show that murine SVZ NPCs and OPCs express the fractalkine receptor (CX3CR1) and bind fractalkine. Exogenous fractalkine directly enhances OPC and oligodendrocyte genesis from SVZ NPCs in vitro. Infusion of fractalkine into the lateral ventricle of adult NPC lineage-tracing mice leads to increased newborn OPC and oligodendrocyte formation in vivo. We also show that OPCs secrete fractalkine and that inhibition of endogenous fractalkine signaling reduces oligodendrocyte formation in vitro. Finally, we show that fractalkine signaling regulates oligodendrogenesis in cerebellar slices ex vivo. In summary, we demonstrate a novel role for fractalkine signaling in regulating oligodendrocyte genesis from postnatal CNS precursor cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
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Oligodendroglía
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Ventrículos Laterales
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Quimiocina CX3CL1
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Células Precursoras de Oligodendrocitos
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Receptor 1 de Quimiocinas CX3C
Límite:
Animals
Idioma:
En
Revista:
Stem Cell Reports
Año:
2021
Tipo del documento:
Article