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Epitope screening of the major capsid protein within grouper iridovirus of Taiwan and the immunoprotective effect with SWCNTs as the vaccine carrier.
Zhang, Zhongyu; Liu, Gaoyang; Liu, Jingyao; Zhu, Bin; Wang, Gaoxue; Ling, Fei.
Afiliación
  • Zhang Z; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China.
  • Liu G; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China.
  • Liu J; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China.
  • Zhu B; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China.
  • Wang G; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China. Electronic address: wanggaoxue@126.com.
  • Ling F; College of Animal Science and Technology, Northwest A & F University, Yangling, 712100, China. Electronic address: feiling@nwsuaf.edu.cn.
Fish Shellfish Immunol ; 117: 17-23, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34280519
Iridovirus can cause a mass of death in grouper, leading to huge economic loss in recent years. At present, practical vaccine is still the best way to control the outbreak of this virus. Many researches had indicated that the major capsid protein (MCP) of grouper iridovirus of Taiwan (TGIV) is an effective antigen to induce a specific immune response in grouper. However, these traditional vaccines that based on large proteins or whole organisms are faced with challenges because of the unnecessary antigenic load. Thus, in this study, we screened the dominant linear epitope within the MCP of TGIV and then, a new peptide vaccine (P2) was developed via prokaryotic expression system. Furthermore, SWCNTs was used as a vaccine carrier to enhance the immunoprotective effect. To evaluate the immunoprotective effect of this vaccine, a total of 245 fish were vaccinated with P2 (5, 10, 20 mg L-1) and SWCNTs-P2 (5, 10, 20 mg L-1) via immersion before being challenged with live TGIV at 28 days post immunization (d.p.i.). Results showed that the serum antibody titer, enzymatic activity, expression level of some immune-related genes (CC chemokine, IgM and TNF-α) and survival rate were significantly increased (SWCNTs-P2, 20 mg L-1, 100%) compared to the control group (0%). These results indicated that this peptide vaccine could effectively induce specific immune response in vaccinated groupers. Functionalized SWCNTs could serve as a carrier of the peptide vaccine to enhance the immunoprotective effect via immersion. To sum up, epitope screening might be a potential way to develop an effective vaccine nowadays, and SWCNTs might provide a practical method that can be used in large-scale vaccination, especially for juvenile fish, to fight against diseases in aquaculture industry.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Perciformes / Portadores de Fármacos / Vacunas Virales / Vacunas de Subunidad / Nanotubos de Carbono / Proteínas de la Cápside / Infecciones por Virus ADN / Enfermedades de los Peces / Iridoviridae / Epítopos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Perciformes / Portadores de Fármacos / Vacunas Virales / Vacunas de Subunidad / Nanotubos de Carbono / Proteínas de la Cápside / Infecciones por Virus ADN / Enfermedades de los Peces / Iridoviridae / Epítopos Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2021 Tipo del documento: Article País de afiliación: China