MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1).
Bioengineered
; 12(1): 8407-8418, 2021 12.
Article
en En
| MEDLINE
| ID: mdl-34657551
ABSTRACT
The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, miR-130a-3p may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of miR-130a-3p in colorectal cancer (CRC) and contributions of Wnt1 pathway modulation, however, have not been examined, hence the exploration on these two aspects. In this study, in comparison with normal controls, both CRC tissue and multiple CRC cell lines showed downregulated miR-130a-3p. MiR-130a-3p overexpression contributed to a decrease in CRC cell proliferation. Additionally, its overexpression also caused reduced expression of WNT Family Member 1 (WNT1) and downstream WNT pathway factors c-myc and cyclin D1. Dual-luciferase assay revealed WNT1 as a direct target of miR-130a-3p, and further the inhibitory effect of miR-130a-3p on c-myc and cyclin D1 was proved to be reversed by overexpressed WNT1. Collectively, miR-130a-3p inhibits CRC growth by directly targeting WNT1, and miR-130a-3p and WNT1 pathway-associated factors are defined as potential targets for CRC treatment.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Regulación hacia Abajo
/
MicroARNs
/
Proteína Wnt1
Tipo de estudio:
Guideline
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Bioengineered
Año:
2021
Tipo del documento:
Article
País de afiliación:
China