Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging.
J Cereb Blood Flow Metab
; 42(6): 966-978, 2022 06.
Article
en En
| MEDLINE
| ID: mdl-34929105
ABSTRACT
Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrß. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their offshoots (arteriole-capillary transition zone), as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Capilares
/
Pericitos
Límite:
Animals
Idioma:
En
Revista:
J Cereb Blood Flow Metab
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos