[The in vivo imaging of zebrafish for chemical vascular toxicity assessment].
Zhonghua Yu Fang Yi Xue Za Zhi
; 56(1): 33-37, 2022 Jan 06.
Article
en Zh
| MEDLINE
| ID: mdl-35092988
ABSTRACT
Objective:
To evaluate the vascular toxicity of chemicals by a real-time observation approach using the transgenic zebrafish.Methods:
The spatiotemporal vascular alterations of transgenic zebrafish after chemical exposure were assessed by laser confocal microscopy and high-content screening analysis, respectively.Results:
The method using Laser Confocal Microscopy (LCM) is easier to operate and yields high-resolution images, while it is lower throughput and inefficient. In contrast, high-content analysis (HCA) analysis obtains high-quality data of vascular toxicity manifesting whole blood vasculature, whereas it requires delicate operation procedures and advanced experimental conditions.Conclusion:
Two kinds of zebrafish imaging methods each have advantages and disadvantages. LCM is suitable for the evaluation of a small number of chemicals. HCA, a cutting-edge technology, has great potential for chemical safety assessment allowing high throughput vascular toxicity tests of a good number of chemicals at a time.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Sistema Cardiovascular
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Año:
2022
Tipo del documento:
Article
País de afiliación:
China