Molecular Characterization of 14-3-3 Zeta Gene in Musca domestica (Diptera: Muscidae) and Its Roles in Response to Bacterial Infection.
J Insect Sci
; 22(5)2022 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-36315471
ABSTRACT
The 14-3-3 gene plays important role in many biological processes, including cell survival, apoptosis, and signal transduction. However, function of the 14-3-3 homologous gene in Musca domestica remains unclear. Here, we identified and characterized the 14-3-3ζ of M. domestica. We found that Md14-3-3ζ gene was highly homologous with other close insects. The qRT-PCR analysis revealed that the Md14-3-3ζ was highly expressed in adults, and was expressed predominantly in hemocytes and fat body. Meanwhile, the expression of Md14-3-3ζ was up-regulated after injecting Escherichia coli and Staphylococcus aureus. Moreover, the recombinant protein rMd14-3-3ζ strongly inhibits the growth of E. coli and S. aureus. Notably, the rMd14-3-3ζ inhibits E. coli and S. aureus by permeating the cell membrane. Taken together, our findings suggested that Md14-3-3ζ is involved in the immune response against bacteria through damaging the cell membrane.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones Bacterianas
/
Muscidae
/
Moscas Domésticas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Insect Sci
Asunto de la revista:
BIOLOGIA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China