Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering.
STAR Protoc
; 4(1): 101905, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36528856
ABSTRACT
A significant number of proteins are annotated as functionally uncharacterized proteins. Within this protocol, we describe how to use protein family multiple sequence alignments and structural bioinformatics resources to design loss-of-function mutations of previously uncharacterized proteins within the glycosyltransferase family. We detail approaches to determine target protein active sites using three-dimensional modeling. We generate active site mutants and quantify any changes in enzymatic function by a glycosyltransferase assay. With modifications, this protocol could be applied to other metal-dependent enzymes. For complete details on the use and execution of this protocol, please refer to Ilina et al. (2022).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bioensayo
/
Ingeniería de Proteínas
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos